Return PythonShell output to a http response

What OS are you running?
Is there a reason you are running as root? Note that it is creating a new flows file, which suggests that you were not running as root previously, or that the hostname has changed (DESKTOP-O493CRT).
What did you do that made it stop working?
Show us the full node-red startup log and tell us what you are putting in the browser as the url.