This should do what you want. It uses a Delay node in Queuing mode to buffer them up and uses the Flush feature of that node to send the next one when the previous is complete. Select the Comment node, and in the Info tab in the right hand pane there will be additional instructions on how to use it. You replace the Function node with your nodes of course.
[{"id":"b6630ded2db7d680","type":"inject","z":"bdd7be38.d3b55","name":"","props":[{"p":"payload"},{"p":"topic","vt":"str"}],"repeat":"","crontab":"","once":false,"onceDelay":0.1,"topic":"","payload":"","payloadType":"date","x":140,"y":840,"wires":[["ed63ee4225312b40"]]},{"id":"ed63ee4225312b40","type":"delay","z":"bdd7be38.d3b55","name":"Queue","pauseType":"rate","timeout":"5","timeoutUnits":"seconds","rate":"1","nbRateUnits":"1","rateUnits":"minute","randomFirst":"1","randomLast":"5","randomUnits":"seconds","drop":false,"allowrate":false,"outputs":1,"x":310,"y":840,"wires":[["d4d479e614e82a49","7eb760e019b512dc"]]},{"id":"a82c03c3d34f683c","type":"delay","z":"bdd7be38.d3b55","name":"Some more stuff to do","pauseType":"delay","timeout":"5","timeoutUnits":"seconds","rate":"1","nbRateUnits":"1","rateUnits":"second","randomFirst":"1","randomLast":"5","randomUnits":"seconds","drop":false,"allowrate":false,"outputs":1,"x":800,"y":840,"wires":[["7c6253e5d34769ac","b23cea1074943d4d"]]},{"id":"2128a855234c1016","type":"link in","z":"bdd7be38.d3b55","name":"link in 1","links":["7c6253e5d34769ac"],"x":95,"y":920,"wires":[["3a9faf0a95b4a9bb"]]},{"id":"7c6253e5d34769ac","type":"link out","z":"bdd7be38.d3b55","name":"link out 1","mode":"link","links":["2128a855234c1016"],"x":665,"y":920,"wires":[]},{"id":"b23cea1074943d4d","type":"debug","z":"bdd7be38.d3b55","name":"OUT","active":true,"tosidebar":true,"console":false,"tostatus":false,"complete":"payload","targetType":"msg","statusVal":"","statusType":"auto","x":670,"y":760,"wires":[]},{"id":"d4d479e614e82a49","type":"debug","z":"bdd7be38.d3b55","name":"IN","active":true,"tosidebar":true,"console":false,"tostatus":false,"complete":"payload","targetType":"msg","statusVal":"","statusType":"auto","x":470,"y":760,"wires":[]},{"id":"3a9faf0a95b4a9bb","type":"function","z":"bdd7be38.d3b55","name":"Flush","func":"return {flush: 1}","outputs":1,"noerr":0,"initialize":"","finalize":"","libs":[],"x":190,"y":920,"wires":[["ed63ee4225312b40"]]},{"id":"7eb760e019b512dc","type":"function","z":"bdd7be38.d3b55","name":"Some functions to be performed","func":"\nreturn msg;","outputs":1,"noerr":0,"initialize":"","finalize":"","libs":[],"x":550,"y":840,"wires":[["a82c03c3d34f683c"]]},{"id":"e35f37deeae94860","type":"comment","z":"bdd7be38.d3b55","name":"Set the queue timeout to larger than you ever expect the process to take","info":"OK, here is a simple flow which allows a sequence of nodes to be protected so that only one message is allowed in at a time. It uses a Delay node in Rate Limit mode to queue them, but releases them, using the Flush mechanism, as soon as the previous one is complete. Set the timeout in the delay node to a value greater than the maximum time you expect it ever to take. If for some reason the flow locks up (a message fails to indicate completion) then the next message will be released after that time.\nMake sure that you trap any errors and feed back to the Flush node when you have handled the error. Also make sure only one message is fed back for each one in, even in the case of errors.","x":270,"y":720,"wires":[]}]