Hi @menno
for future reference, please have a read of this post that explains how to share code in the forum properly.
The HTTP Request node doesn't support providing username/password as part of the url, you have to provide them separately in the node's configuration. You say you've tried the 'use authentication' options, but the flow you've shared doesn't have it enabled.
Edit the node and tick the 'Use authentication' box. Make sure 'basic authentication' is selected, then enter your username and password. Don't forget to remove the values from the url as well.
If that still doesn't work, reexport the HTTP Request node (we don't need the whole flow) and share it here. Any credentials such as username/password will not be included in the export, but we'll be able to see how else the node is configured.