If you mean that you have a section of flow and you want to wait till one message has completely passed through that section before the next one is allowed through then have a look at this example of how to do it. Set the time in the Queue node to a greater time than your flow should ever take to run through. If something locks up then it will release the next message after that time in order to let things keep going.
[{"id":"b6630ded2db7d680","type":"inject","z":"bdd7be38.d3b55","name":"","props":[{"p":"payload"},{"p":"topic","vt":"str"}],"repeat":"","crontab":"","once":false,"onceDelay":0.1,"topic":"","payload":"","payloadType":"date","x":120,"y":1700,"wires":[["ed63ee4225312b40"]]},{"id":"ed63ee4225312b40","type":"delay","z":"bdd7be38.d3b55","name":"Queue","pauseType":"rate","timeout":"5","timeoutUnits":"seconds","rate":"1","nbRateUnits":"1","rateUnits":"minute","randomFirst":"1","randomLast":"5","randomUnits":"seconds","drop":false,"allowrate":false,"outputs":1,"x":290,"y":1700,"wires":[["d4d479e614e82a49","7eb760e019b512dc"]]},{"id":"a82c03c3d34f683c","type":"delay","z":"bdd7be38.d3b55","name":"Some more stuff to do","pauseType":"delay","timeout":"5","timeoutUnits":"seconds","rate":"1","nbRateUnits":"1","rateUnits":"second","randomFirst":"1","randomLast":"5","randomUnits":"seconds","drop":false,"allowrate":false,"outputs":1,"x":780,"y":1700,"wires":[["7c6253e5d34769ac","b23cea1074943d4d"]]},{"id":"2128a855234c1016","type":"link in","z":"bdd7be38.d3b55","name":"link in 1","links":["7c6253e5d34769ac"],"x":75,"y":1780,"wires":[["3a9faf0a95b4a9bb"]]},{"id":"7c6253e5d34769ac","type":"link out","z":"bdd7be38.d3b55","name":"link out 1","mode":"link","links":["2128a855234c1016"],"x":645,"y":1780,"wires":[]},{"id":"b23cea1074943d4d","type":"debug","z":"bdd7be38.d3b55","name":"OUT","active":true,"tosidebar":true,"console":false,"tostatus":false,"complete":"payload","targetType":"msg","statusVal":"","statusType":"auto","x":650,"y":1620,"wires":[]},{"id":"d4d479e614e82a49","type":"debug","z":"bdd7be38.d3b55","name":"IN","active":true,"tosidebar":true,"console":false,"tostatus":false,"complete":"payload","targetType":"msg","statusVal":"","statusType":"auto","x":450,"y":1620,"wires":[]},{"id":"3a9faf0a95b4a9bb","type":"function","z":"bdd7be38.d3b55","name":"Flush","func":"return {flush: 1}","outputs":1,"noerr":0,"initialize":"","finalize":"","libs":[],"x":170,"y":1780,"wires":[["ed63ee4225312b40"]]},{"id":"7eb760e019b512dc","type":"function","z":"bdd7be38.d3b55","name":"Some functions to be performed","func":"\nreturn msg;","outputs":1,"noerr":0,"initialize":"","finalize":"","libs":[],"x":530,"y":1700,"wires":[["a82c03c3d34f683c"]]}]
